吡格列酮对MZT期小鼠胚胎发育阻滞的影响

doi:10.11843/j.issn.0366-6964.2016.11.011

摘要/Abstract

摘要：

本研究旨在了解活性氧（Reactive oxygen species，ROS）、发育阻滞与抗氧化物吡格列酮（Pioglitazone，PIO）之间的关系。试验分3组：对照组、H₂O₂处理组和H₂O₂+PIO处理组。通过H₂O₂建立小鼠胚胎氧化损伤模型，添加抗氧化物质PIO对胚胎进行氧化修复， DCHFDA测定胚胎内部ROS水平，半定量PCR测定母型-合子型胚胎内部相关母性效应基因和合子型基因的表达。结果表明：（1）1-细胞和2-细胞胚胎H₂O₂处理组的ROS水平明显比对照组和H₂O₂+PIO处理组的高（P<0.05）；（2）H₂O₂处理组的2-细胞向4-细胞过渡率明显比对照组和H₂O₂+PIO处理组低（P<0.05），但各处理组之间1-细胞向2-细胞过渡率差异不显著（P>0.05）；（3）H₂O₂处理组的母性效应基因Hsf1、Zfp3612、Zp3和合子型基因Eif1-α、Zscan4d和Muerv-L的表达明显比对照组和H₂O₂+PIO处理组低（P<0.05）。综上表明，氧化应激通过下调2-细胞胚胎母型效应基因和合子型基因表达，导致小鼠胚胎2-细胞发育阻滞，抗氧化物质吡格列酮可促进胚胎发育，有效克服胚胎体外2-细胞发育阻滞现象。

Abstract:

The aim of this study was to investigate the relationship of ROS, developmental block and pioglitazone as an antioxidant. The experiment was divided into 3 groups：Control group, H₂O₂ group and H₂O₂+PIO group. The oxidative damage model of mouse embryos induced by H₂O₂ was established and treated with or without pioglitazone. The ROS level after treatment with pioglitazone was measured by DCHF-DA and the expression of maternal effect genes and zygotic genes were analyzed by Semi-quantitative reverse transcription PCR. The results showed that: (1) the ROS level of H₂O₂group was significantly higher than control group and H₂O₂+PIO group in both 1-cell and 2-cell embryos (P＜0.05); (2) the rate of 2-cell to 4-cell transition in H₂O₂ group was significantly lower than control group and H₂O₂+PIO group (P＜0.05), while the difference of 1-cell to 2-cell transition rate among the groups was not significant (P＞0.05)；(3) the expression of maternal effect genes (Hsf1,Zfp3612,Zp3) and zygotic genes (Eif1-α,Muerv-L,Zscan4d) in H₂O₂ group was lower than control group and H₂O₂+PIO group (P＜0.05). The results provided a clear molecular aspect regarding the mechanism by which pioglitazone promoted embryos development and overcome 2-cell block.

中图分类号:

Q813.7

徐礼杰，李钟淑，方南洙. 吡格列酮对MZT期小鼠胚胎发育阻滞的影响[J]. 畜牧兽医学报, 2016, 47(11): 2240-2247.

XU Li-jie，LI Zhong-shu，FANG Nan-zhu. Effect of Pioglitazone on Development Block of Mouse Embryos during Maternal to Zygotic Transition[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(11): 2240-2247.

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